| iPSC generation and maintenance |
Induced pluripotent stem cells (iPSC) generation and maintenance |
Flora Vaccarino |
| RNA extraction and isolation |
RNA isolation and sequencing |
Flora Vaccarino |
| Neural differentiation |
Neural Differentiation of hiPSCs |
Flora Vaccarino |
| CNON cell culture |
CNON Cell Culture |
Peggy Farnham, James Knowles |
| CNON ChIP-seq |
Histone ChIP and Library Construction Protocol for CNON cells |
Peggy Farnham, James Knowles |
| ATACseq tissue preparation |
Nuclei preparation from frozen tissue for ATAC-Seq (no detergent) |
Gregory Crawford |
| ChIPseq from tissue |
ChIP from frozen tissue |
Nenad Sestan |
| ChIPseq library prep |
ChIPseq library prep |
Schahram Akbarian |
| Total RNA isolation and QC for RNAseq |
RNA isolation and QC |
Chunyu Liu |
| SMRI total RNA isolation and QC |
SMRI RNA isolation and RNA QC |
Chunyu Liu |
| Nuclei isolation and ChIP Protocol |
Nuclei isolation and ChIP |
Schahram Akbarian |
| ATACseq sample and library prep |
ATACseq sample and library preparation, QC, and sequencing |
Chunyu Liu |
| RiboZero stranded library prep |
Ribo Zero stranded library preparation, QC and sequencing |
Chunyu Liu |
| RNAseq data analysis pipeline |
RNAseq data analysis pipeline |
Chunyu Liu |
| ATACseq tissue prep |
Nuclei preparation from frozen tissue for ATAC-Seq |
Gregory Crawford |
| Qiagen kit RNA isolation |
RNA isolation by RNeasy Plus Mini Kit (Qiagen) |
Nenad Sestan |
| IsoSeq library prep |
Isoform Sequencing (IsoSeq) Full-Length cDNA Library Preparation Protocol |
Dalila Pinto |
| LncRNA library prep |
SeqCap lncRNA Enrichment System for Illumina Sequencing: Library Preparation Protocol |
Dalila Pinto |
| MWAC Ab screening |
Rapid screening of antibody collections by MWA |
Chunyu Liu |
| MWAC RPPA |
Microwestern array Reverse phase protein array protocol |
Chunyu Liu |
| MWAC tissue prep |
Microwestern array Tissue Prep Protocol |
Chunyu Liu |
| MWAC Micro Western |
MicroWestern Protocol |
Chunyu Liu |
| Single cell RNASeq |
Single cell RNAseq |
Nenad Sestan |
| RNA from tissue mirVana kit |
Total RNA isolation (including small RNAs) from frozen tissue by miRVANA miRNA kit |
Nenad Sestan |
| Nuclei isolation protocol |
Nuclei isolation protocol for FACS sorted cells |
Stella Dracheva |